Frequent false-positive results ofAspergillus latex agglutination test

Cancer ◽  
1999 ◽  
Vol 86 (2) ◽  
pp. 274-281 ◽  
Author(s):  
Masahiro Kami ◽  
Yoshinobu Kanda ◽  
Seishi Ogawa ◽  
Shin-ichiro Mori ◽  
Yuji Tanaka ◽  
...  
Keyword(s):  
False Positive ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Positive Results

scholarly journals Immunodiagnosis of histoplasmosis in a compromised host

1978 ◽  
Vol 8 (5) ◽  
pp. 558-565
Author(s):  
G A Land ◽  
J H Foxworth ◽  
K E Smith
Keyword(s):  
False Positive ◽  
Inflammatory Diseases ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Serological Tests ◽  
Screening Protocol ◽  
Immunosuppressed Patients ◽  
Yeast Phase ◽  
Positive Results ◽  
Compromised Patients

Three serological tests for the diagnosis of histoplasmosis were compared for sensitivity and specificity in serum from blood bank donors, patients with histoplasmosis, and infected or noninfected immunosuppressed patients. The histoplasmin latex agglutination test was positive in 9% of the normal patients, 33% of the histoplasmosis patients, and 61% of the noninfected immunosuppressed patients. Since the test is prone to many false-positive results in patients with inflammatory diseases or non-Histoplasma infections, it has limited potential as a screening test among compromised patients. Immunodiffusion and counterimmunoelectrophoresis using a mycelial antigen were found to be more sensitive than either test using a combined yeast and mycelial antigen or a pure yeast phase antigen. Counterimmunoelectrophoresis at pH 7.2 proved to be the test of choice for serodiagnosis of histoplasmosis, resolving 85% of the immunocompetent infected patients and 100% of the infected immunosuppressed patients. Results indicated that counterimmunoelectrophoresis in conjunction with immunodiffusion could be used as a screening protocol to determine infection in incoming patients in a cancer hospital.

scholarly journals False positive reactions with rotavirus latex agglutination test.

1992 ◽  
Vol 45 (5) ◽  
pp. 460-460 ◽  
Author(s):  
N Brink ◽  
S Rice ◽  
C Pickering ◽  
S Thurlbeck
Keyword(s):  
False Positive ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
False Positive Reactions

Development of a Latex Agglutination Test for the Rapid Identification of Vibrio parahaemolyticus

1994 ◽  
Vol 57 (1) ◽  
pp. 31-36 ◽  
Author(s):  
TSUNG C. CHANG ◽  
CHI H. CHEN ◽  
HUI C. CHEN
Keyword(s):  
Outer Membrane ◽  
False Positive ◽  
Vibrio Parahaemolyticus ◽  
Outer Membrane Proteins ◽  
False Negative ◽  
Sodium Dodecyl ◽  
Rapid Identification ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test

A latex agglutination test for the rapid identification of Vibrio parahaemolyticus has been developed. Two bacterial outer membrane proteins, with molecular weights of 36,000 and 34,000, were obtained by preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis and were subsequently used as antigens for producing antibodies in rabbits. Immunoelectron microscopy demonstrated that the two proteins were present in the outer membrane of the microorganism. Latex particles sensitized with the affinity-purified antibodies were used as a reagent for the rapid identification of the bacterium. Of 173 strains (including 94 isolates of V. parahaemolyticus, 40 isolates of other vibrios and 39 strains of other bacteria) tested, the false-negative and false-positive rates were 1.4 and 3.1%, respectively. Some strains of three vibrios (V. alginolyticus, V. harveyi, and V. mimicus) produced false-positive results. However, no cross-reactions were observed with the latex reagent among the 39 strains (33 species) of other bacteria. It is proposed that suspicious colonies (green or blue) of V. parahaemolyticus on thiosulfate-citrate-bile salts-sucrose agar (TCBS) be subcultured to tryptic soy agar with 3% NaCl for overnight incubation. Cultures grown on tryptic soyagar-3% NaCl may be used for latex agglutination and galactosidase assays. V. parahaemolyticus will be latex positive and galactosidase negative. Under this condition, V. parahaemolyticus can be identified in only one day after suspect colonies were observed on TCBS agar.

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